Instruments

Instrumentation and services are available to all researchers inside and outside of the university.

Researchers from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but at an external academic or external non-academic rate, which is determined using subsidy and market rates.

All users must register their funds by generating an RRCAN before requesting service or scheduling equipment usage.

All users are responsible for their own laboratory safety training to be current during each use of the core facilities.

AB Sciex QTRAP 5500/6500 and Agilent 1290 UPLC

MRM/SRM Quantitation

 

The AB Sciex QTRAP  is a triple quadrupole mass spectrometer equipped with electrospray (ESI) and atmospheric pressure chemical ionization (ESI+APCI). The mass spectrometer is capable of the following triple quad MS-MS experiments: Selective Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM), Dynamic MRM, product ion scan, precusor ion scan, DIA and neutral loss scan.

The Agilent 1290 series UPLC system consists of refrigerated auto sampler, heated column compartment, gradient LC pumps capable of pressures up to 800 Barr, and photo diode array UV/vis absorbance detector for on-line LC-UV-MS-MS analyses. This is a highly sensitive triple quadrupole instrument that is ideal for high-throughput quantitative analysis of compounds in complex mixtures.

User training is required for use of this instrument.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/5500_QTRAP

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/6500_QTRAP

Agilent 6545  Q-TOF and Agilent 1290 UPLC

Metabolites, natural products

Metabolomics & lipidomics

 

Raising the standard for mid-range Q-TOF instruments, the Agilent 6545 Accurate-Mass Q-TOF LC/MS system offers higher resolving power and sensitivity that is on the order of five times higher than our earlier workhorse instruments. Incorporating the new fast and powerful SWARM autotune feature, the 6545 enables every user to the get the best results—whether your lab is focused on profiling metabolomics or lipidomics, screening food and environment samples, or pharmaceuticals. By combining the power of the 6545 with efficient MassHunter data-mining tools and PCDLs, you can generate answers faster and with greater confidence than ever before.

The Agilent 1290 series UPLC system consists of refrigerated auto sampler, heated column compartment, gradient LC pumps capable of pressures up to 800 Barr, and photo diode array UV/vis absorbance detector for on-line LC-UV-MS-MS analyses. This is a highly sensitive triple quadrupole instrument that is ideal for high-throughput quantitative analysis of compounds in complex mixtures.

The 1260 Infinity II SFC system is an easy-to-use solution for supercritical fluid chromatography. The UHPLC power range helps you to achieve LC-like sensitivity, making this solution ideal for analysis of low-level impurities in chiral and achiral compounds. The system uses beverage-grade CO2, meaning operating costs are 10 to 15-times lower than systems requiring higher purity CO2. Full investment flexibility is guaranteed through the broadest and most versatile range of SFC instrumentation.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/qtof

Agilent 6410 QQQ and Agilent 1260 HPLC

MRM/SRM Quantitation

 

The Agilent QQQ  is an Agilent 6410 triple quadrupole mass spectrometer equipped with electrospray (ESI), Multi mode electrospray and atmospheric pressure chemical ionization (ESI+APCI), Chip Cube interface and atmospheric pressure photo ionization (APPI) sources . The mass spectrometer is capable of the following triple quad MS-MS experiments: Selective Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM), Dynamic MRM, product ion scan, precusor ion scan, and neutral loss scan.

The Agilent 1200 series HPLC system consists of refrigerated auto sampler, heated column compartment, gradient LC pumps capable of pressures up to 600 Barr, and photo diode array UV/vis absorbance detector for on-line LC-UV-MS-MS analyses. This is a highly sensitive triple quadrupole instrument that is ideal for high-throughput quantitative analysis of compounds in complex mixtures.

User training is required for use of this instrument.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/agilentqqq2

Shimadzu IT-TOF LC-MS

Accurate Mass & Structural elucidation

 

The Shimadzu LCMS-IT-TOF is a hybrid mass spectrometer coupling atmospheric pressure ionization with Ion-Trap (IT) and Time-of-Flight (TOF) technologies. It delivers high mass accuracy and high mass resolution independent of MS mode. Resolution is 10,000 at m/z 1000 independent of MS mode. The LCMS-IT-TOF has been designed to maximize sensitivity and selectivity by optimizing the ion transport to the TOF analyzer and redefining the capability of the quadrupole ion trap. The ion trap is used to focus ions before ejection into the TOF as well as supporting MSn analysis with effective precursor ion selection capabilities (resolution > 1,000 at 1,000 m/z).

User training is required for use of this instrument.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/shimadzuittof

JEOL GCMate II Double-Focusing Magnetic Sector GC-MS

EI, CI, FAB M.W., Accurate Mass

 

The JEOL GCMate II is a high resolution double focusing magnetic sector benchtop GC mass spectrometer equipped with ion sources for electron impact (EI), chemical ionization (CI) and fast atom bombardment (FAB) by direct insertion probe and is capable of exact mass measurements for elemental composition determinations. The spectrometer is equipped with high energy Collision Induced Dissociation (CID) linked scan unit allowing MS-MS fragmentation experiments (product ion scans, precursor scans, constant neutral loss scans) for use in structural analysis. The mass spectrometer’s data processing software automatically bridges to the NIST Mass Spectral Database Library for searching unknown compounds.

An Agilent 6890 GC with Electronic Pressure Control and a split/splitless capillary column injector interfaced to the GCMate allows GC/MS by EI or CI for mixture analysis. An Agilent 7673 Automatic Liquid Sampler (ALS) with 100 vial tray attached to the GC allows unattended sample injections.

Mass range is from a low of m/z 1 up to m/z 1000 at full accelerating voltage and up to m/z 3000 at reduced accelerating voltage. Resolution is user selectable from 500 up to 5000. Sensitivity is 30 pg of methyl stearate injected into the GC column producing a S/N of 10:1 at a resolution of 500 for the molecular ion, m/z 298.

 

User training is required for use of this instrument.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/jeolgcmate2

AB Sciex MALDI TOF/TOF 4700

Peptide/intact protein analysis

 

The Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF™ Optics acquires MS and MS/MS spectra in a high-throughput, easy-to-use MALDI platform. It supports a wide variety of front-end sample preparation methods, such as 1-D and 2-D gels, multi-dimensional liquid chromatography (MDLC), or Applied Biosystems proprietary Isotope Coded Affinity Tag technology, as well as back-end bioinformatics platforms. The 4700 Proteomics Analyzer has a coaxial tandem time-of-flight MS/MS configuration providing high-energy collision-induced dissociation for fragmentation with structural information such as immonium ions, side-chain cleavages, and internal fragments. This structural detail can elucidate posttranslational modifications and leucine/isoleucine differentiation and other complexities within high-resolution proteomics. The large mass range and high-energy fragmentation of the TOF/TOF™ optics can analyze proteins without prior digestion. The high rep-rate laser and high-speed data acquisition system provide high sensitivity and dynamic range.

User training is required for use of this instrument.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/abi4700

Thermo Fisher Orbitrap Velos Pro with ETD Agilent nanoHPLC

Global proteomics, Biomarker discovery, Post-translational modifications

Quantitative proteomics (SILAC/TMT/iTRAP)

 

Building on the industry-leading Thermo Scientific Orbitrap platform, the Thermo Scientific Orbitrap Velos Pro hybrid ion trap-Orbitrap mass spectrometer features dual-pressure linear ion trap technology that delivers increased sensitivity and dynamic range with ultimate robustness. With the Thermo Scientific Velos Produal-pressure linear ion trap front end, rapid scanning enables higher productivity and Generation II optics with neutral blocking technology provide enhanced ruggedness for the system. The outstanding high-resolution, accurate-mass (HR/AM) data quality and sensitivity of the Orbitrap Velos Pro™  system, whether using CID, HCD or optional ETD fragmentation, makes it ideal for identification of low-level proteins in complex matrices, rapid quantitation of isobarically labeled peptides and structural elucidation of metabolites.

Nanoflow LC is widely used for high resolution separations and high sensitivity MS detection. The 1260 Infinity Nanoflow LC system incorporates Agilent’s unique Electronic Flow Control (EFC) with active feedback and real-time flow adjustment. EFC delivers a constant flow to the column that is independent of back pressure in the system. Combined with Agilent’s capillary and nanoflow pumps, EFC is the gold standard for reliable and robust nanoflow performance. The 1260 Infinity Nanoflow LC is extremely easy to use. Precise flow rates support stable ion generation to ensure obtain high sensitivity nanoflow LC/MS performance.

https://uicore.rrc.uic.edu/facilities/mmpf/instruments/mmpforbi

Please note, Investigators from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but, at either external academic or external non-academic rate.