Genome Editing Core

Welcome to the Genome Editing Core at UIC! To use the Core's DNA/RNA design and production, mouse or cell line services, go to the Project Initiation page and submit a request. Information about our amplicon sequencing service and sequencing requests can be found on the Sequencing page. Subscribe by email to stay updated when we add new videos, protocols, or services.

Mission

The GEC seeks to enable broad incorporation of genome editing technology and genetically engineered cells and mice into researcher's programs by providing CRISPR reagents, training, and services.

Who we are

The GEC was formed in 2014, sprouting from the incorporation of CRISPR/Cas9 techniques into the lab of Dr. Brad Merrill. The power of CRISPR/Cas9 and its potential to redefine molecular genetic research inspired Dr. Merrill and the UIC Cancer Center to start up a new core with a three-pronged mission of 1) helping labs incorporate CRISPR/Cas9 genome editing into their existing research programs, 2) performing genome editing procedures to generate customized cell lines and mice, and 3) innovating new genome editing technology.

Since 2015, the GEC has been run by Assistant Director Maureen Regan, with oversight by Dr. Merrill. Mrs. Regan has a master’s degree in Chemistry and 7+ years of gene editing experience with technologies including ZFN, TALEN, & CRISPR platforms. She is responsible for all aspects of your project, from design to delivery.

Currently, Cody Hasty is the “lead editor” of the GEC. Mr. Hasty is an UIC undergraduate, and is responsible for assisting with molecular cloning and CRISPR reagent production.

What we do

Experimental Design:

  • Consultation prior to starting a project, providing perspective on what is easy/difficult or low/high efficiency types of procedures.

Generate CRISPR Reagents:

  • sgRNA for your project.
  • donor DNA for precise edits.
  • ssODN for small mutations.
  • Cas9 mRNA.
  • Cas protein.

Generate Edited Cell Lines:

  • new “knockout” cell lines.
  • precise changes to coding sequence.
  • edit noncoding regulatory elements.
  • Provide guidance to users who want to do the cell work in their lab.

Generate Edited Mice:

  • Provide quality controlled sgRNA and Cas9 mRNA for zygote injections.
  • provide donor DNA for precise edits.
  • schedule injection and assist in the transfer of mice from transgenic facility.

Analyze Genome Edited Outcomes:

  • use next-generation sequencing to provide quantitative characterization of all edited alleles in your mouse or cell line project.