The Sequencing Core (SQC) is a shared resource facility that serves the UIC scientific community as as well as external academic and non-academic researchers. The SQC is a multi-faceted facility providing a broad range of services to investigators, including nucleic acid extraction, cell line authentication, Mycoplasma testing, quantitative PCR, capillary sequencing and next-generation sequencing (NGS). The core also offers consultation for all projects, as needed. The core also houses instrumentation for customer use (real-time PCR instruments, nucleic acid quantification devices, and acoustic shearing devices) and provides full service for nucleic acid extraction, quantitative PCR and analysis, daily capillary electrophoresis (Sanger) sequencing, and high-throughput NGS for analysis of DNA and RNA. The facility has particular expertise in the high-throughput microbiome studies and is highly responsive for time-sensitive sequencing projects.
The Sequencing Core (SQC) is a customer-friendly facility dedicated to supporting investigators working on research projects involving nucleic acids. The SQC supports investigators by providing access to high-end instrumentation and to a knowledgeable staff capable of conducting projects from sampling to data analysis. In addition to standard sample processing, SQC staff members assist in project design and planning, manuscript and grant writing, rapid generation of preliminary data, and letters of support. Contact us for more information!
The SQC aims to acquire instrumentation to serve the needs of UIC faculty and researchers, with a focus on next-generation sequencing. Due to rapid changes in NGS sequencing technology, the SQC partners with other facilities to provide users with the most advanced sequencing instrumentation. The SQC is looking to improve automation at the next-generation sequencing library preparation step (i.e. converting total genomic DNA and total RNA to sequencer-ready molecules) to increase throughput and reproducibility.