To request any of the following services, please click here.

Daily Capillary Electrophoresis Sequencing Services

  • Same Day Sequencing Service
  • Standard Sequencing Service
  • Standard Sequencing Service for Ready-To-Load Plates (24-47RXNs)
  • Standard Sequencing Service for Ready-To-Load plates (48-96RXNs)
  • Template Preparation from Culture for Sanger Sequencing (mini-prep)

To request this service, please click here.

Nucleic Acid Extraction, Quantification, and Quality Analysis Services

The SQC has instrumentation and highly trained staff for a wide range of extraction protocols. Instrumentation includes two Promega Maxwells, Qiagen QIACube, and a Qiagen EZ1 extraction robot. Most sample types can be extracted at the SQC, including but not limite to, feces, tissues, cell cultures, FFPE (DNA and RNA), soil, filters, etc. The facility maintains BSL2 laboratories for extraction of BSL2 pathogens and samples with pathogenic potential. Nucleic acids can be assessed for quality and quantity using agarose gel electrophoresis, automated gel electrophoresis using our Agilent TapeStation devices, Nanodrop quantification, Qubit quantification, and Picogreen quantification using a 96-well plate reader.

To request these services, please click here.

Quantitative Analyses of Nucleic Acids

The DNAS facility provides quantitative PCR instruments for customer use (ABI 7500 and ViiA7 instruments) through a registration system in our lab management software, iLABs. In addition, DNAS staff are highly experienced in performing reverse transcription, pre-amplification, assay selection, plate loading, qPCR operation, and data analysis. On a fee-for-service basis, our staff can conduct qPCR projects according to investigator requests. In addition to standard qPCR, the facility also maintains a digital PCR device (Life Technologies QuantStudio3D) for absolute quantification of targets and for measuring allele frequency (for example). Finally, our facility performs extraction-independent RNA sequencing of human cells and tissue (fresh/frozen/FFPE) using the BioSpyder TempO-Seq protocol.

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Fragment Analysis Services

Fragment analyses are performed on our ABI 3730xl DNA Analyzer in a 96-well format. Samples can be provided in water or formamide. We provide internal standards (Only LIZ1200 and LIZ600) for analyses. Please contact our core for details regarding sample submission.

We also perform cell line authentication service using short tandem repeat analysis.

For microbial ecologists, we also provide automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (TRFLP) analyses.

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Amplicon-Based NGS Sequencing for Microbiome Studies

High-throughput next-generation sequencing of short (<520 bp) amplicons is an area of expertise for the DNAS facility. Samples can be processed from genomic DNA or from PCR products (when produced according to facility instructions). Briefly, a two-stage PCR amplification protocol is used to generate amplicons containing Illumina sequencing adapters, a sample-specific barcode sequence, and the amplification target. These amplicons are purified and pooled together and sequenced using paired-end sequencing on an Illumina MiSeq instrument. In general, 20,000-50,000 sequences per sample are generated. A full bioinformatics pipeline for microbial 16S ribosomal RNA gene annotation and analysis has been developed with UIC’s Center for Research Informatics (CRI). See our microbiome pamphlet for more details.

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Visit our Research Informatics Core to learn more about in-depth data analysis services.

Library preparation and sequencing of DNA and RNA templates using NGS platforms

The DNAS facility operates an Illumina NextSeq500 sequencer for high-data sequencing demands, including: genome sequencing/re-sequencing, RNA-seq, ChIP-seq, hybridization target-capture sequencing, shotgun metagenome and metatranscriptome sequencing, and other applications. Library preparation protocols used include DNA-based library preparation protocols including KAPA Hyper, Illumina NeoPrep, Illumina Nextera and Nextera XT, and others. Library preparation protocols for RNA-sequencing are primarily conducted by the Core Genomics facility, and include: Lexogen 3’-mRNA-seq, Lexogen SENSE, Illumina TruSeq, and BioSpyder TempO-Seq. Ribosomal RNA depletion using RiboZero or RiboMinus can be performed as well. Contact directors of DNAS, CG and CRI facilities for discussions on choosing the appropriate sequencing strategy and appropriate sequencing depth and experimental design (particularly number of replicates). Data are generally delivered using Illumina’s BaseSpace cloud storage and analysis environment. The NextSeq500 instrument can generate from 20-150 Gb of sequence data in 75-150 base reads, in single-end or paired-end read mode. For de novo bacterial genome sequencing, DNAS has partnered with external facilities to perform PacBio sequencing, and we have acquired an Oxford Nanopore sequencing device.

To request this service, please click here.

Visit our Research Informatics Core to learn more about in-depth data analysis services.

Please note, Investigators from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but, at either external academic or external non-academic rate.