Services

Instrumentation and services are available to all researchers inside and outside of the university.

Researchers from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but at an external academic or external non-academic rate, which is determined using subsidy and market rates.

 

All users must register their funds by generating an RRCAN before requesting service or scheduling equipment usage.

 

All users are responsible for their own laboratory safety training to be current during each use of the core facilities.

Surface Plasmon Resonance

Description: The real-time detection and monitoring of the biomolecule interactions such as proteins-proteins, proteins-peptides, proteins-DNA, and proteins-compounds. Our Biacore T200 can detect from large protein complexes (> 100 kDa) to a very small fragment compounds (< 100 Da), and it can provide quantitative information on binding specificity, binding affinity (KD), kinetics (ka & kd), and concentration. This technique can also be applied to every stage of drug-discovery, which includes mid-throughput automated compound screening, hit confirmation and validation, hit characterization via kinetics, and mechanism of action.

SPR (Biacore T200) Internal UIC External Academic Non - Academic Unit
Instrument_Biacore T200_self-run $200.00 $250.00 $400.00 1 day
Instrument_Biacore T200_assisted-run $400.00 $500.00 $800.00 1 day
Service_Trouble shooting/Experimental consults $50.00 $62.50 $100.00 1h
Service_SPR Data Analysis $50.00 $62.50 $100.00 1h
Service_Reagent/screening preparation $50.00 $62.50 $100.00 1h
Service_Compound cherry picking $30.00 $37.50 $60.00 0.5h
Training_Biacore T200_1 person $220.00 $275.00 $400.00 1 person
Training_Biacore T200_2 people in a group $150.00 $187.50 $300.00 1 person
Training_Biacore T200_3 people in a group $130.00 $162.5 $260.00 1 person
Training_Biacore T200_4 people in a group $115.00 $143.75 $230.00 1 person
Training_Biacore T200_5 people in a group $100.00 $125.00 $200.00 1 person
Training_SPR Data Analysis_Basic $50 $62.50 $100 1h
SPR Research project collaboration Contact Hyun Lee (danielhl@uic.edu)

Other Biophysical Instruments

Description: Understanding the function and structure of biomolecules is crucial in all areas of modern health and life sciences. Characterizing the structure and dynamics of these molecules is essential to understanding the basis of various cellular function, and the Biophysics Core at the Center for Structural Biology is to provide unimpeded access to state-of-the-art and well-maintained biophysics equipment.

Other Instruments Internal UIC External Academic Non-academic Unit
Instrument_AUC $390.00 $500.00 $787.5 per run
Instrument_CD $25.00 $31.25 $50.00 1 hour
Instrument_Emulsiflex $11.25 $14.06 $22.50 1 hour
Instrument_Sonicator $11.25 $14.06 $22.50 30 min
Instrument_ITC $20.00 $25.00 $45.00 1 hour
Instrument_DLS $20.00 $25.00 $25.00 1 hour
Instrument_AKTA purifier FPLC $30.00 $37.50 $60.00 1 hour
Instrument_AKTAxress FPLC $45.00 $62.50 $90.00 1 hour
Instrument_PerkinElmer_Victor3V plate reader $20.00 $25.00 $45.00 1 hour
Instrument_Centrifuge_Sorvall LYNX 4000 $10.00 $12.50 $20.00 30 min
Training_CD $75.00 $95.00 $150.00 1-2 hours
Training_Emulsiflex $50.00 $62.50 $100.00 30 min
Training_Sonicator $50.00 $62.50 $100.00 30 min
Training_DLS $50.00 $62.50 $100.00 30 min
Training_ITC $100.00 $125.00 $200.00
Training_AUC $250.00 $312.50 $500.00 1 person
Training_AKTA purifier $150.00 $187.50 $300.00 1-2 hours
Training_AKTAxpress $175.00 $200.00 $350.00 ~2 hours
Training_ PerkinElmer_Victor3V plate reader $50.00 $62.50 $100.00 30 min
Training_ Data Analysis_CD, DLS, ITC $50.00 $62.50 $100.00 1 hour
Training_ Data Analysis_AUC $100.00 $125.00 $200.00 1-2 hours
User Assistance $50.00 $62.50 $100.00 1 hour
Research projects Contact Hyun Lee (danielhl@uic.edu)

Protein over-expression and Purification

Description: Large-scale protein purification service is offered to users who need purified proteins for SPR, High-Throughput Screening (HTS), and structural biology (X-ray crystallography and NMR). AKTAxress FPLC enables us to purify four different proteins at a time using a well optimized two-step (HisTrap and Size-exclusion columns) purification protocol.

Protein Purification (Itemized services) Unit cost ($) External Academic Non-academic Unit
Transformation into DH5a (or XL1) cells_Option $50.00 $62.50 $100.00 1 plasmid
Transformation into Rosetta2 (DE3) or BL21 (DE3) cells_Option $50.00 $62.50 $100.00 1 plasmid
2L culture_Protein purification_Affinity column (Ni-NTA_HisTrap)_Basic $1,000.00 $1,250.00 $2,000.00 1 purification
Protein purification_tag-cleavage_reload (Ni-NTA)_Option $250.00 $312.50 $500.00 1 purification
TEV_protease_Option $50.00 $62.50 $100.00 1 purification
SUMO_protease_Option $50.00 $62.50 $100.00 1 purification
Protein_purification_Extra_column_Ion-exchange_Option $250.00 $312.50 $500.00 1 purification
Protein_purification_Extra_column_Size-Exclusion (Superdex 75)_Option $350.00 $437.50 $600.00 1 purification

Macromolecular Structural Biology

Biophysics core will assist research projects needing macromolecular structure determination and the biophysical characterization of proteins in collaboration using X-ray crystallography (at Argonne) and Cryo-EM (at University of Chicago). Please contact Hyun Lee (danielhl@uic.edu).