Genome Research Core

Genome Research Core is a combination of three sub-units - genomics, sequencing, and genome editing.

The Genome Editing (GE) sub-unit was formed in 2014, sprouting from the incorporation of CRISPR/Cas9 techniques into the lab of Dr. Brad Merrill. The power of CRISPR/Cas9 and its potential to redefine molecular genetic research inspired Dr. Merrill and the UIC Cancer Center to start up a new core with a three-pronged mission of 1) helping labs incorporate CRISPR/Cas9 genome editing into their existing research programs, 2) performing genome editing procedures to generate customized cell lines and mice, and 3) innovating new genome editing technology.

Since 2015, the GE has been run by Assistant Director Maureen Regan, who has a master’s degree in Chemistry and years of gene editing experience with technologies including ZFN, TALEN, & CRISPR platforms. She is responsible for all aspects of your project, from design to delivery.

The Sequencing (SQ) sub-unit serves the UIC scientific community as as well as external academic and non-academic researchers as a multi-faceted facility providing a broad range of services to investigators, including nucleic acid extraction, cell line authentication, Mycoplasma testing, quantitative PCR, capillary sequencing and next-generation sequencing (NGS). It also offers consultation for all projects, as needed. Sequencing also houses instrumentation for customer use (real-time PCR instruments, nucleic acid quantification devices, and acoustic shearing devices) and provides full service for nucleic acid extraction, quantitative PCR and analysis, daily capillary electrophoresis (Sanger) sequencing, and high-throughput NGS for analysis of DNA and RNA. It has particular expertise in the high-throughput microbiome studies and is highly responsive for time-sensitive sequencing projects.

Notice to all Research Resources Center Customers

In order to minimize COVID-19 spread, the Research Resources Center, Genome Research Core is implementing the following temporary mandatory guidelines for using this core:

  • All customers entering the core space must wear new, clean Gloves will be provided if you do not have your own
  • If there is a need to remove gloves, wash your hands with soap frequently and for at least 20 seconds. Do not touch your face or other individuals. Replace gloves as soon as possible
  • Instead of shaking hands, exchange greetings with a wave, bow, or nod
  • When using real-time PCR or Nanodrop instruments, gloves must be worn
  • Try to keep at least one meter/three feet distance between yourself and others
  • Limit the number of people in shared space rooms (g., A310) to two at a time
  • Disinfect anything you have used during your visit with 70% ETOH after every use. Disinfectant will be provided adjacent to instrumentation
  • We are temporarily cancelling group training sessions for real-time PCR instrumentation. Please contact facility staff for more information (grc@uic.edu)

If you have any flu like symptoms (e.g., fever, cough, shortness of breath) please do not use the core services. You should stay home and follow the appropriate guidelines as indicated by the Illinois Department of Public Health (IDPH) – http://www.dph.illinois.gov/topics-services/diseases-and-conditions/diseases-a-z-list/coronavirus.