Your browser is unsupported

We recommend using the latest version of IE11, Edge, Chrome, Firefox or Safari.

RRC will observe the UIC reduced service schedule for nonessential services. Core facilities will close at 5p on Thursday, Dec. 22, and will reopen at 8:30a on Tuesday, Jan. 3.

Services

Instrumentation and services are available to all researchers inside and outside of the university.

Researchers from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but at an external academic or external non-academic rate, which is determined using subsidy and market rates.

 

All users must register their funds before requesting service or scheduling equipment usage.

 

All users are responsible for their own laboratory safety training to be current during each use of the core facilities.

Mass Spectrometry Core provides a range of services Heading link

  • Exact Mass Analysis for small molecules and peptides
  • Protein molecular weight measurement
  • Protein/peptide fractionation
  • Protein identification
  • Global quantitative proteomics by label-free or labeling methods (TMT, iTRAQ, SILAC)
  • Post-translational modification (PTM) analysis – phosphorylation, acetylation, oxidation, histone acetylation
  • Metal analysis by ICP MS
  • Untargeted metabolomics/lipidomics
  • Targeted protein/peptide quantification by MRM
  • Targeted metabolomics/lipidomics by MRM
  • Instrument Training on Qtrap, Orbitrap, MALDI-TOF, Q-TOF and ICP MS
  • Method Development on request
  • Bile acids
  • Ceramide- Molecular species
  • Ceramide-1-Phosphate (C1P)
  • Sphingosine – Molecular species (S1P)
  • Eicosanoids (prostaglandins, leukotrienes, etc)
  • Endocannabinoids (eCBs)
  • Free fatty acids
  • Short chain fatty acids (SCFA)
  • Lysophosphatidic acid (LPA) – Molecular species
  • Phosphatidic acid (PA) – Molecular species
  • Cholesterol and Oxysterols
  • Glycolipids – GM1, GM2, GM3, GT1, GD1
  • Phosphatidylinositols (PI, PIP, PIP2, PIP3)
  • Sex hormones – Testosterone (T, DHT), estrogen (E1, E2, E3), cortisol, cortisone
  • Amino Acids
  • Nucleotide and Deoxynucleotide
  • Perfluorooctanoic acids
  • Catecholamines
  • Acyl-CoA
  • Polyfluoroalkyl substances (PFAS)
  • cGAMP
  • Antibiotics
  • Herbicides/pesticides
  • Primary aromatic amines (PAA)
  • Flux

 

URL Name
Please visit the iLab page to view more details or place an order for services, link here.

Service Pricing Heading link

ServiceDescriptionPricing (Internal)
S01-Bile Acid Analysis
Quantitative analysis of bile acids in biological samples 

TCDCA
TDCA
GCDCA
UDCA
CA
TLCA
GLCA
CDCA
DCA
GCA

This cost covers a section of 35min Qtrap LC-MS run for data acquisition, a section of 10min labor time and a section of reagent kit 2. 

Additional fee will be applied if extra sample preparation is needed.
$60.00
S02-Creatinine Measurement in urine

Creatinine measurement for urine samples.$10.00
S03-ECM proteomics
A part of ECM proteomics pipeline including MSC and RIC cores.

For MSC core service, the cost covers a section of 2 hours gradient, a section of Mascot database search and a section of Scaffold analysis.

Additional fee will be applied from RIC core.

Additional fee will be applied if extra sample preparation is needed.
$160.00
S04-Exact Mass by MALDI-TOFMALDI TOF MS analysis. Supplied matrices including SA, DI, CHCA, DHB and HPA. Other uncommon matrices will be provided by users with special needs.

The cost covers a section of 5min instrument time, 5min labor time and a section of reagent kit2.
$20.00
S05-Exact Mass or MS/MS Analysis by LC/MS on Q-TOFPerform MS or MS/MS analysis by LC/MS.

MS and MS/MS analysis can be conducted for one sample with additional 50% cost.

The cost covers a section of a 15min LC/MS gradient on Qtof 6545, a section of 10min of labor time and a section of reagent kit 2.
$30.00
S06-Herbicide AnalysisAssay 1
Glyphosate
aminomethyl-phosphonic acid (AMPA)
glufosinate (GLU)
3-methylphosphinico-propionic acid (MMPA)

Assay 2
Atrazine
desethylatrazine (DEA)
desisopropylatrazine (DIA)
diaminochlorotriazine (DACT)



The cost covers a section of 10min Qtrap LC/MS run, a section of 5min labor time and a section of reagent kit2.

Each assay will be counted as one service.

Additional fee will be applied if extra sample preparation is needed.
$30.00
S07-Histone AnalysisCommon Histone (H2, H3, H4, etc.) protein modification quantitative analysis
The cost covers a section of sample digestion, a section of desalting, a section of derivatization, a section of 2-hour HF MS data acquisition, a section of Mascot database search and a section of one-hour labor.
$400.00
S08-Instrument Training on ICPInstrument training on ICP MS.

Up to 2 trainees are allowed for each section. The second trainees from the same research group needs to pay additional $450 for the 8-hr instrument time in order to gain access.
$800.00
S09-Instrument Training on MALDI-TOFInstrument training on MALDI-TOF$640.00
S10-Instrument Training on QtofInstrument training on Agilent Qtof 6545.

8hrs of assisted instrument time is included.

Up to 2 trainees are allowed in each section. The 2nd trainees from the same research group needs to pay additional $500 for the 8-hr instrument time in order to gain access.
$900.00
S11-Instrument Training on QtrapInstrument training on Qtrap. Up to 2 trainees are allowed for each section. The second trainees from the same research group needs to pay additional $450 for the 8-hr instrument time in order to gain access. $800.00
S12-Instrument Training on Velos ProInstrument training on Orbitrap Velos Pro.

8hrs of assisted instrument time is included.

Up to 2 trainees are allowed in each section. The 2nd trainees from the same research group needs to pay additional $600 for the 8-hr instrument time in order to gain access.
$1,000.00
S13-MRM quantitation methodMethod development of MRM;based quantitation, including LC and MS optimization of the targeted small molecule and generation of a calibration curve

The cost covers a section of 3-hour LC/MS run, a section of 2 hour labor.
$300.00
S14-MRM quantitation method proteinMethod development of; based quantitation, including protein digestion, LC and MS optimization of the targeted protein and generation of a calibration curve.

The cost covers a section of 3-hour LC/MS run, a section of 2 hour labor, a section of desalting, a section of protein digestion and 4 sections of reagent kit 2.
$450.00
S15-MRM Standard CurveStandard curve (8 points) used in each batch of MRM analysis

The cost covers a section of 120min LC/MS run, a section of 1-hour labor and a section of reagent kit 2.
$200.00
S16-Nucleoside Modification AnalysisAdenosine and M6A analysis.

Analysis of Guanine, Uridine and other bases or nucleosides are also available.

The cost covers a section of 10min LC/MS run, a section of 10min labor and a section of reagent kit 1.
$25.00
S17-Peptide fractionationPeptide fractionation by high pH reversed-phase HPLC. Default use 2 hour gradient for 60 fractions.

The cost covers a section of 2-hr HPLC run, a section of 1-hour labor, 4 section of reagent kit 2 and 3 sections of speed vac.
$200.00
S18-PFAS analysisPFOA/PFOS analysis by MRM (EPA 537.1 or 8327)

The cost covers a section of 20-min LC/MS run, a section of 10-min labor and a sectoin of reagnet kit 1.
$40.00
S19-Protein ID without fractionationProtein identification service

The cost covers a section of protein digestion, a section of 1-hr nano-LC/MS run, a section of desalting, a section of Mascot search and a section of scaffold analysis.
$175.00
S20-Protein/compound Molecular Weight DeterminationProtein or compound molecular weight measurement by high resolution mode of Q-Tof or QE-HF coupled to UPLC.

The cost covers a section of 40-min LC/MS run, a section of 10min labor and a section of reagent kit 1.
$60.00
S21-S1p AnalysisQuantitative analysis of Sphingosine-1-phosphate (S1p) in biological samples.

The cost covers a section of 25-min Qtrap LC/MS run, a section of 5-min labor and a section of reagent kit 1.
$40.00
S22-Short Chain Fatty Acid QuantificationSCFA quantitative analysis in biological samples including fecal, serum/plasma, cell lysate, etc.
Routine analysis covers C2-C5. Other SCFAs are also available.

The cost covers a section of derivatization, a section of Qtrap 25-min LC/MS MRM run, a section of 5min labor time and a section of reagent kit 1.

Additional charge will be applied if extra sample preparation is needed.
$55.00
S23-Trace Metal Analysis by ICP MSDigested solution for metal analysis

Standard suite includes Al V Cr Mn Fe Ni Co Cu Zn As Se Mo Cd Sb Sn Pb
The cost covers a section 7min ICP MS run, a section of 5min labor and a section of reagent kit 1.
Additional fee will be applied if acid digestion is needed.
$18.00
S24-Untargeted Metabolomics/lipidomicsUntargeted metabolomics/lipidomics analysis by; Q-TOF MS. Run with positive and negative modes with RP column. All the features detected by MS will be aligned, normalized and annotated by Agilent software.

The cost covers a section of 50min LC/MS run, a section of 10min labor and a section of reagent kit 2.

Additional statistical analysis will be provided for fee if requested.
$90.00
S25-Sex Hormones quantificationSex hormones and related metabolites quantification such as Estrone (E1), Estradiol (E2), 2-hydroxyestradiol, 2-methoxyestradiol,Progesterone, Allopregnanolone, Testosterone, Dihydrotestosterone etc.

The cost covers a section of derivatization, a section of 30min Qtrap LC/MS run, a section of 5min labor and a section of reagent kit1.

Additional fee will be applied if sample preparation is needed.
$60.00
S26-Tryptophan Cycle analysisMetabolites quantification related to tryptophan cycle such as tryptophan, kynurenic acid, kynurenine, serotonin, dopamine, norepinephrine etc.

The cost covers a section 25min Qtrap LC/MS run, a section of 5min labor and a section of reagent kit 1.

Additional charge will be applied if sample preparation is needed.
$40.00
S27-Customized AnalysisThe following service are also available in the core. The price will be determined by the number of analytes, sample types, sample preparation etc. Please contact the core for more details. $0.00
Targeted assayCeramide
Ceramide-1-Phosphate (C1P)
Eicosanoids (prostaglandins, leukotrienes, etc)
Endocannabinoids (eCBs)
Free fatty acids
Lysophosphatidic acid (LPA) – Molecular species
Phosphatidic acid (PA) – Molecular species
Cholesterol and Oxysterols
Glycolipids – GM1, GM2, GM3, GT1, GD1
Phosphatidylinositols (PI, PIP, PIP2, PIP3)
Amino Acids
Nucleotide and Deoxynucleotide
Catecholamines
Acyl-CoA
cGAMP
Antibiotics
Primary aromatic amines (PAA)
Flux
Protein or peptide quantification
Untargeted assayGlobal quantitative proteomics by TMT, iTraq or SILAC.