Research Histology and Tissue Imaging Core

The mission of the Research Histology and Tissue Imaging Core (RHTIC) is to provide high throughput histological and digital microscopy imaging services for reproducible, automated analysis of biomarkers expression levels in human and animal tissues as well as for measurements of spatial and temporal features of biological objects. The Research Histology and Tissue Imaging Core (RHTIC) is located on the 6th floor of the College of Medicine Research Building (Rooms 6100 and 6094).  The core is supported and managed collaboratively by the Research Resource Center and the Department of Pathology since 2011.

The Histology lab is a fully functioning, state-of-the-art research facility providing the following services:

  • Tissue processing, embedding, and sectioning (FFPE, frozen samples, cell pellets and 3D cell cultures)
  • Direct chemical stains (e.g., H&E, trichrome, Picrosirius red, toluidine blue, and others)
  • Brightfield immunohistochemistry for up to two targets
  • Immunofluorescence of 1-3 simultaneous fluorochromes using standard detections systems
  • TSA Immunofluorescence of up to 6 targets with PerkinElmer Opal dyes

The RHTIC offers two major digital microscopy systems:  the Aperio Scanscope AT2®for whole-slide brightfield imaging and the PerkinElmer Vectra® 3 for multispectral imaging in brightfield or fluorescence imaging.  Available software for image analysis includes Definiens Tissue Studio®, Leica Aperio Image Analysis Framework, PerkinElmer inForm® and Indica Labs Halo.®

Imaging services provided include the following:

  • Brightfield whole slide digital scans of fixed or frozen tissue or cytological samples at 20x or 40x magnification.
  • Visualization and annotation of slides with a free image viewer (Aperio ImageScope®)
  • Image analysis using machine learning and stain quantification
  • Analysis of tissue microarrays (TMAs)
  • Analysis of multiplexed targets in a single slide using conventional fluorescent dyes or Opal®tyramide signal amplification technology (PerkinElmer) at 10x, 20x and 40x magnification

Core users can bring stained slides for scanning, or have their slides stained at the facility. Please schedule a consultation visit to discuss new projects with Maria Sverdlov, Core Director (, tel: 312-355-0177).