General Guidelines For Sample Submission to The Histology Core
- Label cassettes in #2 pencil or the one that writes dark.
- Place tissue into cassettes and close cassettes securely. Please make sure the tissue is small enough to move within the cassette (no thicker than a nickel). If it is too large you might need to dissect it into smaller pieces for it to fix properly. Also please remove sutures prior to fixation.
- Tissue needs to be fixed immediately. Place cassettes into a container large enough to allow the cassettes to move around and fill it with 10% neutral buffered formalin (10%NBF) or 4%PFA. Please use at least 15-20 volumes of formalin for every volume of tissue.
- Fix tissues at room temperature for 48hr.
- After fixation, remove formalin and replace it with 70% ethanol.
- Bring your samples to the Histology core in 70% ethanol in a SEALED container (The core no longer accepts samples in inappropriate containers). If you don’t have the right container, please ask us, and we will try to help you find one.
- Please make a typed list of all your samples and bring it with you to the Histology Core (COMRB 6100).
- We will walk you through the submission process to be sure you are getting everything you need.
- Please allow 2 weeks (although it can take longer) for your order to be processed. If you need it sooner, please let us know – there will be an additional RUSH charge applied for the expedited service.
Users of the facility are required to acknowledge use of the facility in publications or if significant scientific input was provide to include facility members as co-authors. An example of an appropriate acknowledgment may be: “Histology/Imaging services were provided by the Research Resources Center - Research Histology and Tissue Imaging Core at the University of Illinois at Chicago.”