DNA-old
The DNA Services (DNAS) facility is a multi-faceted facility providing nucleic acid extraction, quantitative PCR, sequencing and next-generation sequencing (NGS) services to UIC. The core houses instrumentation for customer use (quantitative PCRs, nucleic acid quantification devices, and acoustic shearing devices) and provides full service for nucleic acid extraction, quantitative PCR and analysis, daily capillary electrophoresis (Sanger) sequencing, and high-throughput NGS for analysis of DNA and RNA.
The facility also provides cell line authentication through STR analysis. The facility has particular expertise in the high-throughput microbiome studies and is highly responsive for time-sensitive sequencing projects. The facility serves UIC researchers, as well as external academic and non-academic researchers. The facility shares instrumentation and collaborates closely with UIC’s Core Genomic (CG) facility. The facility director, Dr. Stefan J. Green, is a molecular biologist and microbial ecologist with over 20 years of research experience, including 5 years as director of the DNAS facility.
The DNAS facility has seven major areas of research activity, including:
- Nucleic acid extraction
- Quantitative analyses of nucleic acids
- Daily capillary electrophoresis sequencing
- Cell line authentication and Mycoplasma testing
- Amplicon-based NGS sequencing for microbiome studies
- Reagent sales for quantitative PCR and oligonucleotide synthesis
- Library preparation and sequencing of DNA and RNA templates using NGS platforms