(cryo)FIB/SEM across the disciplines
A hands-on UIC-NU workshop
March 22 - 23, 2023
9:00 AM - 5:00 PM
Location
Student Center East Illinois Rooms
Address
750 S. Halsted Street, Chicago, IL 60607
Cost
Free
Calendar
Download iCal File
This 2-day workshop will celebrate the arrival of a state-of-the-art FIB/SEM at the UIC’s Research Resources Center Electron Microscopy Core (EMC). The dual-beam instrument, a ThermoFisher Helios 5 CX, was purchased with support from the University of Illinois Chicago, Northwestern University, and the ARO DURIP Program. It is equipped with an Aquilos cryo-stage and a Leica vacuum cryo transfer system, and offers STEM, EDS, and EBSD detectors in addition to back scattered and secondary electron contrast analyses. A unique capability is the tie in with a Ferrovac UHV cryo-shuttle that enables transfer of samples for analysis at the Northwestern University Center for Atom Probe Tomography (NUCAPT).
With this workshop, we aim to bring together current and future users from the Chicagoland metropolitan area and beyond. We will introduce new sample preparation, (quantitative) imaging, and tomography capabilities, and discuss room and cryogenic applications across the physical sciences, engineering, and the life sciences. We will further highlight opportunities for correlative imaging at Chicagoland user facilities such as EMC, NUCAPT, NUANCE, APS/ANL, and the University of Chicago’s Advanced Electron Microscopy facility.
On the first day, the workshop will feature a keynote and invited speakers drawn from world-renowned experts in (cryo)-FIB/SEM, cryo-EM, and atom-probe tomography: Jamie Warner, PhD, University of Texas at Austin; Nabil Basim, PhD, McMaster University; Elizabeth Wright, PhD, University of Wisconsin Madison; Phillip Heck, PhD, Field Museum Chicago; Si Chen, PhD, Argonne National Laboratory; and Derk Joester, PhD, Northwestern University. We will seek contributed and vendor talks representing the broad range of user communities. The second day is reserved for lab-tours and hands-on demos. Tours of UIC-EMC and demos of liquid cell EM capabilities will also be offered to interested workshop participants. Enabling remote participation is under consideration.
Date posted
Feb 8, 2023
Date updated
Mar 20, 2023
Event Schedule Heading link
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Day 1
8:00 AM
Registration and Continental Breakfast – Sponsored by Leica
9:00 AM
Welcome and Opening Remarks (Robert Klie, Derk Joester and UIC Vice Chancellor for Research Joanna Groden)
9:15 AM
Keynote Lecture: Studying full battery coin cells using cryo-FIB and cryo- TEM using a complete air-free system to preserve intrinsic buried interface structure (Jamie Warner, UT Austin)
10:00 AM
Coffee Break
10:30 AM
Morning Session – Biological and Biomaterials
10:30 AM
Elizabeth Wright (UW-Madison): New developments in correlative cryo-EM technologies to support in situ structural biology
11:00 AM
Seth Villarreal (Leica): Like Riding a Bicycle: The Hub and Spoke Workflows of the VCT500
11:15 AM
Satchal Erramilli (University of Chicago): Antibody-based reagents for imaging small proteins by cryo-EM
11:30 AM
Si Chen (ANL – Invited): Explore the use of FIB of high-resolution x-ray microscopy
12:00 PM
Lunch & Lab Tour
1:30 PM
Afternoon Session 1 – Hard Materials
1:30 PM
Nabil Bassim (McMaster University): Recent Advances in using FIB Technology for Materials Research
2:00 PM
Rick Passey (ThermoFisher): Introduction to the Helios 5 CX Gallium DualBeam with Advanced Cryo Sample Preparation
2:15 PM
John Farrell (UIC): Thin Film Materials Characterization using FIB/SEM and atomic-resolution STEM
2:30 PM
Paul Smeets (NU): Making the most out of your ions and electrons: Sample preparation of mineralized tissues with unique shapes and their characterization using advanced STEM techniques
2:45 PM
Philipp Heck (Field Museum/UChicago): New insights from some of the Solar System’s oldest materials in Chicago.
3:15 PM
Coffee Break
3:45 PM
Afternoon Session 2 – Cryo-Characterization
3:45 PM
Derk Joester (NU): Interfaces and Interphases in Mineralized Tissues
4:15 PM
Tera Lavoie (University of Chicago): UChicago’s New EM Facility
4:30 PM
Francisco Lagunas (UIC): Cryo-EM of beam sensitive materials
4:45 PM
End of workshop and possible lab tours
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Day 2
9:00 AM
Demos
Helios 5CX with Leica Cryo-Transfer demo
Topics and samples: TBD
Liquid cell and TEM/STEM lab demos
Topics and samples: TBD
12:00 PM
Casual Lunch
Demos
1:30 PM
Helios 5CX with Leica Cryo-Transfer demo
Topics and samples: TBD
1:30 PM
Liquid cell and TEM/STEM lab demos
Topics and samples: TBD
4:30 PM
Wrap up
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Parking
Parking for this event is available in the Halsted Street Parking Structure, located at 760 W. Taylor Street.
Parking will be validated for attendees when they check in at the registration desk. Attendees must bring their parking ticket with them to be validated.