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RRC will observe the UIC reduced service schedule for nonessential services. Core facilities will close at 5p on Thursday, Dec. 22, and will reopen at 8:30a on Tuesday, Jan. 3.

Services

Sectioning Heading link

  • Paraffin sections
  • Paraffin step sections
  • Frozen sections
  • Curls for DNA/RNA
  • Slides for DNA/RNA
  • Slides for laser capture microdissection

H&E and Special Stains Heading link

  • Trichrome
  • Toluidine Blue
  • Periodic Acid-Schiff (PAS)
  • Sirius Red / Fast Green
  • Oil Red O (Lipid Stain)
  • Alcian Blue
  • Others can be optimized per request

Immunohistochemistry (IHC) and Immunofluorescence (IF) Heading link

  • Single and Dual IHC (HRP/AP)
  • Single, dual and triple IF using various fluorescent dyes (Alexa Flours etc…)

PerkinElmer Tyramide Signal Amplification Heading link

  • 2-6 targets plus Dapi
  • Can use antibodies of the same species (all mouse, all rabbit)
  • Stable signal over time
  • Can image in the light

Brightfield Whole Slide Imaging – Leica Aperio AT2 Heading link

  • 20x and 40x magnifications
  • Whole tissues and tissue microarrays
  • Free Leica Aperio Imagscope Software

Fluorescent Imaging – PerkinElmer Vectra 3 Heading link

  • IHC or TSA
  • Multispectral imaging with autofluorescence subtraction
  • Can separate 7+ dyes

Image Analysis Heading link

  • PerkinElmer inForm
  • Indica Labs Halo
  • Definiens Tissue Studio
  • ImageJ/Fiji